• Ben Elf as Nemo
  • The Wise Old Elf as Marlin
  • Mrs. Elf as Coral
  • Holly Thistle as Dory
  • Pob (from Pob's Programme) as Blenny the Worried Fish
  • Big Jet (from Little Einsteins) as The Anglerfish
  • Toublesome Trucks (from TTTE) as The Seagulls
  • Barnaby Elf as Jauques
  • King Thistle as Gill
  • Queen Thistle as Peach
  • Mr. Elf as Bubbles
  • Murdoch (from TTTE) as The Whale
  • Farmer (from Shaun the Sheep) as Krill
  • Shirley (from Shaun the Sheep) as Mr. Ray
  • Robbie Rotten (from LazyTown) as Dr. Phillip Sherman
  • Flain (from Mixels) as Squirt
  • Diesel (from TTTE) as The Barracuda
  • Big Bad Barry as Bruce
  • Anti-Holly Anti-Thistle as Darla Sherman
  • Biff Tannen (from Back to the Future) as Anchor
  • Jack Spicer (from Xiaolin Showdown) as Chum
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