Cast[]
- Miss Spider as Twilight Sparkle
- Shimmer as Fluttershy
- Pansy as Pinkie Pie
- Dora (from Dora the Explorer) as Applejack
- Snowdrop as Rainbow Dash
- Kai-Lan (from Ni Hao, Kai-Lan) as Rarity
- Milli (from Team Umizoomi) as Sunset Shimmer
- Molly (from Bubble Guppies) as Starlight Glimmer
- Oona (from Bubble Guppies) as Trixie Lulamoon
- Deema (from Bubble Guppies) as Derpy Hooves
- Zooli (from Bubble Guppies) as Lyra
- Alicia (from Go, Diego, Go!) as Bon Bon
- Tasha (from The Backyardigans) as Octavia
- Uniqua (from The Backyardigans) as Vinyl Scratch
- Squirt as Spike
- Dragon as Flash Sentry
- Bounce as Shining Armor
- Wiggle as Dr. Whooves
- Spinner as Cheese Sandwich
- Holley as Big Macintosh
- Spiderus as Discord