Cast
- Fergy Fudgehog (from Viva Piñata) as Marlin
- Twilight Sparkle as Coral
- Spike as Nemo
- Pinkie Pie as Dory
- Shining Armor as Gill
- Flash Sentry as Bloat
- Applejack as Peach
- Doctor Whooves as Gurgle
- Derpy Hooves as Bubbles
- Rarity as Deb
- Fluttershy as Flo
- Rainbow Dash as Jacques
- Apple Family and Background Ponies as The School of Moonfish
- Pipsqueak as Squirt
- Braeburn as Crush
- Big MacIntosh as Mr. Ray
- Fillies as Kid Fishes
- Discord as Bruce
- The Diamond Dogs as Anchor and Chum
- The Changelings as The Seagulls
- King Sombra as Dr. Philip Sherman
- Trixie as Darla