• Skunk as Mr. Conductor (RS)
  • Frog as Mr. Conductor (GC)
  • Fox as Stacy Jones
  • Rabbit as Schemer
  • Tails (from Sonic) as Matt
  • Fifi La Fume (from Tiny Toon Adventures) as Tanya
  • Pig as Dan
  • Duck as Kara
  • Crane as Becky
  • Tiger as Harry Cupper
  • Panda as Billy Twofeathers
  • Mr. Garrison (from South Park) as JB King
  • Dragon as Mr. Conductor's Evil Twin 
  • Baboon as Buster the Bully
  • Scratch and Grounder (from Sonic) as Bull and Biff
  • Danforth Drake and Roderick Rat (from Tiny Toon Adventures) as The Evil Workers
  • Elmyra Duff (from Tiny Toon Adventures) as Vickie
  • Snake as Mr. Nicholas
  • The Dora the Explorer Characters as The Thomas Characters
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