- Greg as Flik
- Dorothy the Dinosaur as Princess Atta
- Young Nala (from The Lion King) as Dot
- Hannah (from Toy Story) as The Queen
- Keith Urban as Mr. Soil
- Flora Door as Dr. Flora
- Sam Moran as Thorny
- Keith Urban as Cornelious
- Captain Hook (from Peter Pan) as Hopper
- Captain Feathersword as Molt
- Shere Khan (from The Jungle Book) as Thumper
- Sharky and Bones (from J&TNLP) as Axel and Loco
- Paul The Cook as PT Flea
- Murray as Francis
- Wags the Dog as Slim
- Jeff as Heimlich
- Anthony as Dim
- Emily (from Thomas And Friends) as Gypsy
- Henry the Octopus as Manny
- Magdelena the Mermaid as Rosie
- Donald and Douglas (from Thomas And Friends) as Tuck and Roll
- Mighty Dragon (from Angry Birds) as The Bird