Cast
- Mario as Woody
- Luigi as Buzz Lightyear
- Sonic (from Sonic the Hedgehog) as Mr. Potato Head
- Diddy Kong as Slinky Dog
- Yoshi as Rex
- Donkey Kong as Hamm
- Princess Peach as Bo Peep
- Soldier (from Team Fortress 2) as Sarge
- Scout, Pyro, Demoman, Heavy, Engineer, Medic, Sniper, and Spy (from Team Fortress 2) as Sarge's Soldiers
- Baby Mario as Andy Davis
- Baby Peach as Molly Davis
- Rosalina as Mrs. Davis
- Bowser Jr. as Sid Phillips
- Pauline as Hannah Phillips
- Petey Piranha as Scud
- Dorrie as R.C.
- Toads as Squeeze Toy Aliens
- Chunky Kong as Rocky Gibraltar
- Lumas as Billy, Goat, and Gruff
- Baby Yoshi as Mr. Spell
- Nabbit as Robot
- King Boo as Mr. Shark
- Baby Bowser as Etch
- Captain Toad as Lenny
- Koopa Troopas as Red Monkeys
- Cappy as Mike
- Goomba as Hockey Puck
- Poochy as Snake
- Plessie as Little People Fire Truck
- Tiny Kong as Dolly
- Funky Kong as Teddy
- Kiddy Kong as Duckie
- Professor E. Gadd as Roly Poly Clown
- Baby Rosalina as Troll
- Gordon Freeman (from Half-Life) as Combat Carl
- Chell (from Portal)/Zoey (from Left 4 Dead) as Janie/Pterodactyl
- Witch (from Left 4 Dead) as Babyface
- Spitter (from Left 4 Dead) as Legs
- Tank (from Left 4 Dead) as Rockmobile
- Boomer (from Left 4 Dead) as Ducky
- Jockey (from Left 4 Dead) as Walking Car
- Turret (from Portal) as Frog
- Charger (from Left 4 Dead) as Jingle Joe
- Smoker (from Left 4 Dead) as Hand-In-The-Box
- Hunter (from Left 4 Dead) as Roller-Bob
- Announcer (from Team Fortress 2) as Burned Rag Doll
- GLaDOS (from Portal) as Huge Red Pickup Truck Toy
- Francis and Bill (from Left 4 Dead) as Yellow Soldier Toys
- Ms. Pauling (from Team Fortress 2) as Sally Doll