Cast[]
- Fix-It Felix Jr. as Professor Hershel Layton
- Q*Bert as Luke Triton
- Deanna as Flora Reinhold
- Gene as Inspector Chelmey
- King Candy/Turbo as Don Paolo
- Sergeant as Emmy Altava
- Zangief as Dimitri Allen
- Clyde as Bill Hawks
- Paperboy as Barton
- General Hologram as Curtis O'Donnell
- Turtle as Inspector Clamp Grosky
- Roy as Marco Brock
- Wreck-It Ralph as Dr. Andrew Schrader
- Root Beer Tapper as Jean Descole
- Candlehead as Claire Foley
- Rancis as Finch
- Mary as Ameila Ruth
- Sour Bill as Clive Dove
- Wynchel as Pierre Starbuck
- Duncan as Oswald Whistler
- Vanellope as Melina Whistler
- Sonic as Frederick Bargland
- Jubilleena Bing-Bing as Nina
- Bowser as Clark Triton
- Pac-Man as Anthony Herzen
- Dr. Eggman as Desmond Sycamore