- Plex as Batman/Bruce Wayne
- Toodee as Robin/Dick Grayson
- Foofa as Batgirl/Barbara Gordon
- Natasha Fatale (from The Adventures of Rocky and Bullwinkle) as Poison Ivy
- Muno as Two Face
- Brobee as The Riddler
- DJ Lance Rock as The Penguin
- Franklin's Mother {from Franklin} as Catwoman
- Boris Badenov (from The Adventures of Rocky and Bullwinkle) as The Joker
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